Chemistry 426 Study Guide 3

1. List the factors that one must be aware of when doing fluorimetry, and explain how, if ignored, the factor will affect the quantum yield, i.e. the observed signal.

                    2. Explain how a Michelson interferometer works.

3. Describe the advantages of Fourier transform over sequential and multi-channel detection methods.

4 How do atomic emission and atomic absorption differ and because of the difference(s) what changes are required in the instrumentation for the two processes.

5.  Explain the result on the signal observed in an atomic absorption system when each (one at a time) of the parameters that an operator can control is increased. (NOTE: There are at least 7 parameters so the item is not trivial.)

6. Contrast atomic absorption instrumentation with atomic emission (flame, DCP, and ICP) instrumentation.

7. Explain the advantages and disadvantages of DCP and ICP emission over AA absorption analytical methods.

           8. Sketch the signal path in an AA instrument.

        9. Explain why the amount of atomization is critical for reproducibility in atomic spectroscopic methods and          explain what variables affect degree of atomization.

        10. Describe the common causes of spectral and chemical interferences in atomic spectroscopy and                describe the strategies currently in use to correct for these interferences.

        11. Explain basic sample handling regimens for metal analysis.

        12. Sketch the signal path in an emission instrument. e.g. DCP, ICP

13. Explain why (what causes) the requirements of a DCP or ICP monochromator to be more stringent than for an AA monochromator.